Liver subcellular fractions
PRIMACYT Cryopreserved animal
hepatocytes
The acquisition of animal tissue is performed under permits 3902-046-2017 and DE 13 076 0001 21 from the Landkreis Ludwigslust-Parchim, Department of Veterinary- and Foodstuff Control, following EU Regulation No. 1029/2009, Art. 17 and 23 and permit 7221.3-2-004/17 from the Ministry for Agriculture, Food Safety and Fishery of the State Mecklenburg-Pomerania in accordance with EU Regulation 2010/63/EU, and with several additional regulations for the protection of animals (e.g., TierSchG dated July 18, 2016, and TierSchVerV dated August 31, 2015) used for experimentation and other scientific purposes.
PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation. Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.
High quality – High Viability – Characterized and validated.
PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation. Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.
Features and Benefits
- Convenience for the laboratory
Convenient for our customers. Animal Hepatocytes are ready to use. They show high viability and perfectly match your schedules and workflow. - Start to work immediately
Start immediately with your project and do not to wait for liver tissue. Thaw and use hepatocytes where and whenever you need them. - Same quality as for human hepatocytes
Our animal hepatocytes are carefully prepared by using isolation and cryopreservation methods and tools that are also applied for fresh human hepatocytes. - Long time availability
Cryopreserved hepatocytes can be stored for years at predefined conditions in the vapor phase of liquid nitrogen or in freezers at temperatures below -135°C. Available when you need them, they are an ideal (appropriate) solution for sequential experiments over time.
Application Areas
- Use cryoheps as about fresh ones
Most applications can be performed as using fresh hepatocytes in general considering that cryopreserved cells can be used following a limited number of thawing processes. - Drug development and lead candidate screening.
- Identification of species-specific metabolism activities and toxicity of compounds.
- Studies of drug metabolism and toxicology, including performing transporter activities and coupled phase I and II metabolism studies.
- Prediction of metabolic clearance.
- Assessment of tetracycline-induced steatosis.
- Determination of microsome and hepatocyte scaling factors for in vitro/in vivo extrapolation.
- Drug-drug interaction assays.
- Drug transporter inhibition.
- Drug transporter inhibition.
- Metabolic profiling and assessment of stability.
- Comparison of major phase I and phase II metabolism reactions.