Cryopreserved Common Carp Hepatocytes

Pooled Fish Hepatocytes – a new in-vitro model metabolic System.

Sweetwater fish like Common carp (Cyprinus carpio) have been used in vivo by researchers for decades to assess bioaccumulation, metabolism, and environmental risks of chemicals, including herbicides, crop protection products, and fertilizers. Apart from Rainbow trout, Common carp is one of the best-studied fish species.

The OECD released on June 25, 2018 their Test Guideline 319A, Determination of in vitro intrinsic clearance using cryopreserved rainbow trout. PRIMACYT now offers cryopreserved Common carp hepatocytes prepared following this guideline.

PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading company in the field of research of human and animal hepatocytes. We offer fresh and cryopreserved animal hepatocytes in suspension and plated.

PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) since 2013 and serves as member of the laboratory network responsible for the method validation.

Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.

Animal Tissue Performance and Regulations. The acquisition of animal tissue is performed under permits 3902-046-2017 and DE 13 076 0001 21 from the Landkreis Ludwigslust-Parchim, Department of Veterinary- and Foodstuff Control, following EU Regulation No. 1029/2009, Art. 17 and 23 and permit 7221.3-2-004/17 from the Ministry for Agriculture, Food Safety and Fishery of the State Mecklenburg-Pomerania in accordance with EU Regulation 2010/63/EU, and with several additional regulations for the protection of animals (e.g., TierSchG dated July 18, 2016, and TierSchVerV dated August 31, 2015) used for experimentation and other scientific purposes.

Tust M., Kohler M., Lagojda A., and Lamshoeft M: Comparison of the in vitro assays to investigate the hepatic metabolism of seven pesticides in Cyprinus carpio and Oncorhynchus mykiss. 
https://doi.org/10.1016/j.chemosphere.2021.130254
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Bischof I., Köster J., Segner H., and Schlechtriem C.:  Hepatocytes as in vitro test system to investigate metabolite patterns of pesticides in farmed rainbow trout and common carp: Comparison between in vivo and in vitro and across species. DOI 10.1016/j.cbpc.2016.05.003 Access the publication

Following the OECD guideline 319A Primacyt now offers cryopreserved primary hepatocytes from Common carp kept under controlled conditions for at least two weeks in continuous flow water. Each lot is comprehensively tested for high viability and Cytochrome P450/Phase I activities and is suitable for at least 24 hours in suspension assays. For the first time, plateable cryopreserved carp hepatocytes are made available for researchers worldwide.

This info refers to the product data below:

• Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
• Viability: Denotes post thaw results using the thawing protocol for hepatocytes.
• Plateability in 2D format is tested on collagen coated 24well and 96well plates, for 3D spheroid cultures ultra-low attachment plates Biofloat™ from faCellitate are used.
• To print lot information, please select the print icon at the end of the lists below. For research use only.
• Not intended for human or animal diagnostic or therapeutic use.

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This info refers to the product data below:

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