Cryopreserved Chicken Hepatocytes

Chicken hepatocyte cultures have been used to study lipid metabolism and food additives that may facilitate and improve chicken growth, animal production, and carcass traits. In recent years, chicken hepatocytes in co-cultures with non-parenchymal liver cells (Kupffer cells) also became an essential tool for investigating environmental stress factors like heat stress and mycotoxins in food and feed safety studies. 

More recently, in vitro studies have demonstrated that hepatocytes, microsomes, and hepatic S9 fractions are effective and efficient tools to predict the in vivo endpoint for biotransformation and accumulation testing.

Animals like chicken have been used in vivo by researchers for decades to assess bioaccumulation, metabolism, and environmental risk of chemicals, including drugs, xenobiotics, cosmetics, fragrances, flavouring substances, herbicides and crop protection products.

Our competencies:

PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading company in the field of research of human and animal hepatocytes. We offer fresh and cryopreserved animal hepatocytes in suspension and plated.

PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) since 2013 and serves as member of the laboratory network responsible for the method validation.

Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.

Animal Tissue Performance and Regulations. The acquisition of animal tissue is performed under permits 3902-046-2017 and DE 13 076 0001 21 from the Landkreis Ludwigslust-Parchim, Department of Veterinary- and Foodstuff Control, following EU Regulation No. 1029/2009, Art. 17 and 23 and permit 7221.3-2-004/17 from the Ministry for Agriculture, Food Safety and Fishery of the State Mecklenburg-Pomerania in accordance with EU Regulation 2010/63/EU, and with several additional regulations for the protection of animals (e.g., TierSchG dated July 18, 2016, and TierSchVerV dated August 31, 2015) used for experimentation and other scientific purposes.

Mu Y., Sun L., Leng Z., Cao Y., Li H., and WangY.: Effect of IGFBP2 Overexpression on the Expression of Fatty Acid Synthesis Genes in Primary Culture Chicken Hepatocytes. J. Poult. Sci. 56: 177-185, 2019, doi:10:2141/jpsa.0180114

Chen F., Hou L., Zhu L., Yang C.,  Zhu F., Qiu, H., and Qinx S.: Effects of selenide chitosan sulfate on glutathione system in hepatocytes and specific pathogen-free chickens. Poultry Science 99: 3979–3986, 2020, https://doi.org/10.1016/j.psj.2020.04.024 

Mackei M., Molnár A., Nagy S., Pál L., Kovágó C., Gálfi P., Dublecz K., Husvéth F., Neogrády Z., and Mátis G.: Effects of Acute Heat Stress on a Newly Established Chicken Hepatocyte—Nonparenchymal Cell Co-Culture Model. Animals, 10: 409; 2020, doi:10.3390/ani10030409

Mackei M., Orbán K., Molnár A., Pál L., Dublecz K., F. Husvéth F., Neogrády Z., and Mátis G.: Cellular Effects of T-2 Toxin on Primary Hepatic Cell Culture Models of Chickens. Toxins 12: 46; 2020, doi:10.3390/toxins12010046 

PRIMACYT offers certified cryopreserved chicken (Novogen Brown Light) hepatocytes from pooled and single donors. Pooled hepatocytes are obtained from several individual animal liver perfusions performed in parallel.

Each pool and the individual single donors are comprehensively tested for viability, plateability, suspension use and Cytochrome P450/Phase I activities.

This info refers to the product data below:

• Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
• Viability: Denotes post thaw results using the thawing protocol for hepatocytes.
• To print lot information, please select the print icon at the end of the lists below. For research use only. 
• Not intended for human or animal diagnostic or therapeutic use.

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This info refers to the product data below:

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