Rainbow trout S9-fractions: intestine, gill, brain

Pooled Rainbow trout S9 fractions – a new in-vitro model System.

Animals like Rainbow trout (Oncorhynchus mykiss) have been used in vivo by researchers for decades to assess bioaccumulation, metabolism, and environmental risk of chemicals, including drugs, xenobiotics, cosmetics, fragrances, flavoring substances, herbicides and crop protection products.

Currently, bioaccumulation studies are either performed in living animals or in pooled hepatocytes or liver S9 fractions from Rainbow trout (Oncorhynchus mykiss). The liver is the major organ for detoxification of chemicals. Therefore, in accordance with OECD test guideline 319, hepatocytes and S9 fractions serve as model systems to assess bioaccumulation of foreign materials in fish.

However, organs like gill or intestine also play an important role in uptake, metabolism and excretion of environmental toxins. In addition, toxins may cause serious damage to other organs like e. g. brain. Beside liver S9 fractions from rainbow trout PRIMACYT now offers also S9 fractions from gill, intestine and brain. This will give researches additional test systems for a more precise evaluation of the bioaccumulation of foreign compounds in fish, without the need of expensive and more time-consuming animal experiments.  

PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading company in the field of research of human and animal hepatocytes. We offer fresh and cryopreserved animal hepatocytes in suspension and plated.

PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) since 2013 and serves as member of the laboratory network responsible for the method validation.

Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.

Rainbow Trout – a new in-vitro System

More recently, researchers of the US Environmental Protection Agency (EPA), DuPont Haskell, the University of Bern and Fraunhofer IME have introduced trout liver S9 fractions as a reliable in-vitro system for bioaccumulation and clearances studies. 

Measurements of biotransformation rates in primary fish subcellular fractions can be scaled up to the whole animal and thereby can provide data used in computational models to predict chemical bio-concentration factors in animals (K.A. Faye et al. 2014, I. Bischof et al. 2016). 

These and other supporting research results lead to the implementation of OECD Test Guidelines 319A and 319B on the determination of intrinsic clearance using cryopreserved rainbow trout hepatocytes and liver S9 subcellular fractions.

Access the webpage for this guideline and download the document:

Download Guideline

PRIMACYT now offers rainbow trout S9 subcellular fractions from liver, gill, intestine and brain. Each lot is comprehensively tested for Cytochrome P450/Phase I and Phase II activities.

This info refers to the product data below:

• Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
• Characterization: Denotes assays that have been performed to characterize the products.
• To print lot information, please select the print icon at the end of the lists below. For research use only.
• Not intended for human or animal diagnostic or therapeutic use.

Follow this link to contact us for direct Questions or inquire about your Project information. Thank you.

This info refers to the product data below:

Download MSDS