Turkey liver subcellular fractions

Animal health plays an important role not only for the welfare of pets and domestic farm animals but also for animals used in food production. For the development of veterinary drugs we offer liver derived products from a variety of species including dog, horse, sheep, goat, pig, poultry and fish.

A number of these animals also play an important role in environmental risk assessment of herbicides and fungicides used in crop protection. Isolated hepatocytes and liver subcellular fractions from ruminants, poultry and fish have increasingly become an important research tool in metabolism and bioaccumulation studies. Cost-saving test methods using hepatocyte cultures and subcellular fractions as alternatives to animal experiments will reduce the number of animal experiments and are currently introduced into OECD test guidelines.

PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading company in the field of research of human and animal hepatocytes. We offer fresh and cryopreserved animal hepatocytes in suspension and plated.

PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) since 2013 and serves as member of the laboratory network responsible for the method validation.

Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.

Animal Tissue Performance and Regulations. The acquisition of animal tissue is performed under permits 3902-046-2017 and DE 13 076 0001 21 from the Landkreis Ludwigslust-Parchim, Department of Veterinary- and Foodstuff Control, following EU Regulation No. 1029/2009, Art. 17 and 23 and permit 7221.3-2-004/17 from the Ministry for Agriculture, Food Safety and Fishery of the State Mecklenburg-Pomerania in accordance with EU Regulation 2010/63/EU, and with several additional regulations for the protection of animals (e.g., TierSchG dated July 18, 2016, and TierSchVerV dated August 31, 2015) used for experimentation and other scientific purposes.

Toxicologist are using liver fractions like microsomes from turkey and other birds as model systems to study in vivo metabolism, bioaccumulation and the effects of agrochemicals and fungicides on biotransformation since more than 40 years. Turkey liver subcellular fractions have been use to analyze metabolism, biotransformation and toxic effects of, e.g., mycotoxins, pharmaceuticals, and antibiotics.

Developing regulatory activities (e.g., REACh) require bioaccumulation to be assessed for thousands of chemicals. On top of this, there is increasing pressure to reduce, refine and replace animal tests. This leads to an urgent need for the evaluation of in vitro systems to provide significant data for the estimation of bioaccumulation of chemicals of all kind in human and animals.

More recently, researchers of the US Environmental Protection Agency (EPA), DuPont Haskell, the University of Bern and Fraunhofer IME have validated trout liver S9 fractions as a reliable in-vitro system for bioaccumulation and clearances studies. 

Further reading:

Murcia H, Cruz A, Leon J, Gonzales-Curbelo MA: Study on the inhibitory effect of furafylline and troleandomycin in the 7-methoxyresorufin-O-demethylase and nifedipine oxidase activities in hepatic microsomes from four poultry species using high-performance liquid chromatography coupled with fluorescence and ultraviolet detection. J Pharm Biomed Anal, 2019, 164:148-154

Rawal S, Coulombe RA Jr: Metabolism of aflatoxin B1 in turkey liver microsomes: the relative roles of Cytochromes P450 1A5 and 3A37. Toxicol Appl Pharmacol, 2011: 254:349-354

Murcia HW, Díaz GJ, Cepeda SM: Enzymatic activity in turkey, duck, quail and chicken liver microsomes against four human cytochrome P450 prototype substrates and aflatoxin B1. J Xenobiotics, 2011, 1:e4: 17-21 

Diaz GJ, Murcia HW, Cepeda SM: Bioactivation of aflatoxin B1 by turkey liver microsomes: responsible cytochrome P450 enzymes. Brit Poult Sci, 2010, 828-837

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