Beagle liver subcellular fractions

The liver fulfills many vital processes in mammals. It is the central organ of energy metabolism, responsible for the maintenance of the blood sugar level and the synthesis of plasma proteins under physiological and pathophysiological conditions.

Hepatocytes are the most prominent cells within the liver. Hepatocytes eliminate toxic substances from the blood. In this biotransformation process transporter proteins (influx and efflux transporter), phase I reactions (cytochrome P450 proteins), phase II reactions (mainly glucuronidation and sulfatation) play a central role.

Subcellular liver fractions are a simple but easy to use model system to study Phase I and Phase II reactions, perfectly suited for in vitro metabolism and toxicity / detoxification studies prior to preclinical or clinical tests.

PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading company in the field of research of human and animal hepatocytes. We offer fresh and cryopreserved animal hepatocytes in suspension and plated.

PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) since 2013 and serves as member of the laboratory network responsible for the method validation.

Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.

In drug development, at least two in vivo repeated-dose toxicity studies of a drug candidate have to be performed in two different animal species before human studies can be initiated (https://www.fda.gov/downloads/drugs/guidancecomplianceregulatoryinformation/guidances/ucm292340.pdf). In most cases these species are mouse or rat and dog or monkey.

Hepatotoxicity is a major cause for failure in drug development. For interpretation of hepatotoxicity data, it is important to understand potential interspecies differences in mechanisms that lead to drug toxicity. This includes

– drug uptake into the liver,

– biotransformation of the drug (Phase I and Phase II), and

– drug efflux from the liver

Interspecies differences in expression and activities in transport proteins and biotransformation enzymes may result in different intra- and extrahepatic concentrations of the drug.

Subcellular liver fractions are a simple model system for the analysis of drug biotransformation reactions. They are also used for biodegradation and bioaccumulation studies of chemicals like e. g. herbicides or pesticides.

Beagle liver subcellular fractions are isolated from livers obtained from male or female dogs. Donor demographics stating vaccination status and health status of the animals are available for each animal.

PRIMACYT offers certified cryopreserved Beagle subcellular fractions (S9 and microsomes) from single and pooled donors. Each lot is comprehensively tested for Cytochrome P450/Phase I and Phase II activities.

This info refers to the product data below:

• Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
• Characterization: Denotes assays that have been performed to characterize the products.
• To print lot information, please select the print icon at the end of the lists below. For research use only.
• Not intended for human or animal diagnostic or therapeutic use.

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