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Cryopreserved Beagle Dog Hepatocytes from PRIMACYT.

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    Overview
    The liver fulfills many vital processes in mammals. It is the central organ of energy metabolism, responsible for the maintenance of the blood sugar level and the synthesis of plasma proteins under physiological and pathophysiological conditions.

    Hepatocytes are the most prominent cells within the liver. Hepatocytes eliminate toxic substances from the blood. In this biotransformation process transporter proteins (influx and efflux transporter), phase I reactions (cytochrome P450 proteins), phase II reactions (mainly glucuronidation and sulfatation) play a central role.

    Cryopreserved primary hepatocytes are perfectly suited for in vitro metabolism and toxicity / detoxification studies prior to preclinical or clinical tests.

    Dog hepatocytes are isolated from livers obtained from male or female Beagle and are cryopreserved directly after isolation. Donor demographics stating vaccination status and health status of the animals are available for each animal. Cryopreserved Beagle hepatocytes are used in suspension assays and plated in 2D and 3D cell cultures.
    Our Competencies
    PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading company in the field of research of human and animal hepatocytes. We offer fresh and cryopreserved animal hepatocytes in suspension and plated.

    PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation.

    Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.
    Literature
    In drug development, at least two in vivo repeated-dose toxicity studies of a drug candidate have to be performed in two different animal species before human studies can be initiated (https://www.fda.gov/downloads/drugs/guidancecomplianceregulatoryinformation/guidances/ucm292340.pdf). In most cases these species are mouse or rat and dog or monkey.

    Hepatotoxicity is a major cause for failure in drug development. For interpretation of hepatotoxicity data, it is important to understand potential interspecies differences in mechanisms that lead to drug toxicity. This includes
    - drug uptake into the liver,
    - biotransformation of the drug (Phase I and Phase II), and
    - drug efflux from the liver

    Interspecies differences in expression and activities in transport proteins and biotransformation enzymes may result in different intra- and extrahepatic concentrations of the drug.

    Quantification of drug transporter protein by LC/MS has revealed significant interspecies differences in the expression of uptake and efflux transporters. On the other hand, in beagle hepatocytes expression of the two most important Organic Anion Transporters Oatp1a2 and Oatp1b4 (equivalent to human OATP1B1 and OATP1B3) was maintained at the same level as in dog liver tissue (Wang et al. 2014).

    Wang L, Prasad B, Salphati L, Chu X, Gupta A, Hop C E.C.A., Evers R, and Unadkat J. D.: Interspecies Variability in Expression of Hepatobiliary Transporters across Human, Dog, Monkey, and Rat as Determined by Quantitative Proteomics, Drug Metab. Dispos., 2015, 43:367–374
    Our offering
    Primacyt offers certified pools of cryopreserved Beagle hepatocytes and hepatocytes from single donors. Pooled hepatocytes are obtained from 3 individual animal liver perfusions performed in parallel. After quality control, a pool consisting of equal numbers of viable liver cells from each donor is cryopreserved. In addition, hepatocytes from individual donors are cryopreserved for reference purposes.

    Each pool and the individual single donors are comprehensively tested for viability, plate-ability, Cytochrome P450/Phase I and Phase II activities to ensure that all donors are represented equally within the pool.

    Cryopreserved hepatocytes from individual donors are also tested for drug transporter activities.


    Additional Product Info
    This info refers to the product data below:

    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the thawing protocol for hepatocytes.
    • To print lot information, please select the print icon at the end of the lists below. For research use only.
    • Not intended for human or animal diagnostic or therapeutic use.
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    This info refers to the product data below:




    PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation.
    Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.

    Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.



    PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading company in the field of research of human and animal hepatocytes. We offer fresh and cryopreserved animal hepatocytes in suspension and plated.
    The liver fulfills many vital processes in mammals. It is the central organ of energy metabolism, responsible for the maintenance of the blood sugar level and the synthesis of plasma proteins under physiological and pathophysiological conditions.

    Hepatocytes are the most prominent cells within the liver. Hepatocytes eliminate toxic substances from the blood. In this biotransformation process transporter proteins (influx and efflux transporter), phase I reactions (cytochrome P450 proteins), phase II reactions (mainly glucuronidation and sulfatation) play a central role.

    Cryopreserved primary hepatocytes are perfectly suited for in vitro metabolism and toxicity / detoxification studies prior to preclinical or clinical tests.

    Dog hepatocytes are isolated from livers obtained from male or female Beagle and are cryopreserved directly after isolation. Donor demographics stating vaccination status and health status of the animals are available for each animal. Cryopreserved Beagle hepatocytes are used in suspension assays and plated in 2D and 3D cell cultures.
    The liver fulfills many vital processes in mammals. It is the central organ of energy metabolism, responsible for the maintenance of the blood sugar level and the synthesis of plasma proteins under physiological and pathophysiological conditions.

    Hepatocytes are the most prominent cells within the liver. Hepatocytes eliminate toxic substances from the blood. In this biotransformation process transporter proteins (influx and efflux transporter), phase I reactions (cytochrome P450 proteins), phase II reactions (mainly glucuronidation and sulfatation) play a central role.

    Cryopreserved primary hepatocytes are perfectly suited for in vitro metabolism and toxicity / detoxification studies prior to preclinical or clinical tests.

    Dog hepatocytes are isolated from livers obtained from male or female Beagle and are cryopreserved directly after isolation. Donor demographics stating vaccination status and health status of the animals are available for each animal. Cryopreserved Beagle hepatocytes are used in suspension assays and plated in 2D and 3D cell cultures.




    Product Code BHCP-I-Pool. Cryopreserved Platable Beagle Hepatocytes - Certified Pool - Order Information.

    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the standard thawing protocol.
    • To print lot information, please select print icon at the end of this list.
    LotGenderInventoryViability %Total cells x10exp6/vialViability %Recovery x10exp6/vialTotal number of cells available x10exp6CYP 1A1/2 induction x-foldPrice EUR per Vial
    BH170718-4M738610,2896,6481,8171016.00

    Product Code BHCP-I-Pool
    Price per Vial is determined by the number of cells according to the table below:
    4 - 5.9 Mio - EUR 677.00
    6 - 9.9 Mio - EUR 1,016.00

    Quantity:


    Get in Dialog with PRIMACYT and request a Quote by preformatted Email.

    .



    Download Lot Information



    Product Code BHCP-I-T. Cryopreserved Platable Beagle Hepatocytes for Use in Induction and Transporter Studies - Order Information.
    Information about CYP - Activities and Uptake Transporter Activities are contained in the Lot-Info data, if available.


    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the standard thawing protocol.
    • To print lot information, please select print icon at the end of this list.
    LOTGenderInventoryViability %Total cells x10exp6 /vialViability %Recovery x10exp6 /vialTotal number of cells available x10exp6EROD Inducton (xfold)Price EUR/Vial
    BH130510-1-1M17710,3727,0723,4435,00
    BH130510-1-2M108710,4716,26242,5435,00
    BH130802M258410,0667,51883,6435,00
    BH140616-1F58710,0754,82419,8290,00
    BH140616-2F48910,0664,61819,6290,00
    Q
    This table is sortable by column. Click on the white arrows starts sorting the desired field. Click on the top left arrow resets data to original state.

    Product Code BHCP-I-T
    Price per Vial is determined by the number of cells according to the table below:
    2 - 3.9 Mio - EUR 145.00
    4 - 5.9 Mio - EUR 290.00
    6 - 9.9 Mio - EUR 435.00

    Quantity:


    Get in Dialog with PRIMACYT and request a Quote by preformatted Email.

    .


    Download Lot Information






    Product Code BHCP-I. Cryopreserved Platable Beagle Hepatocytes for Use in Induction Studies - Order Information.
    Information about CYP - Activities are contained in the Lot-Info data, if available.
    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the standard thawing protocol.
    • To print lot information, please select print icon at the end of this list.
    Product Code / LotGenderInventoryViability %Total cells x106/vialViability %Recovery x10exp6/vialTotal number of cells available x106EROD induction x-foldPrice EUR / Vial
    BHCP-I/BH150917M149210,00826,79322339,00
    BHCP-I/BH170907-2F109410,008866027339,00
    BHCP-I/BH180321-1F418910,0084624655339,00
    BHCP-I/BH180417-1M138210,00896,88846339,00
    BHCP-I/BH180417-2M129010,50934,95850226,00
    Q
    This table is sortable by column. Click on the white arrows starts sorting the desired field. Click on the top left arrow resets data to original state.

    Product Code BHCP-I
    Price per Vial is determined by the number of cells according to the table below:
    2 - 3.9 Mio - EUR 113.00
    4 - 5.9 Mio - EUR 226.00
    6 - 9.9 Mio - EUR 339.00

    Quantity:


    Get in Dialog with PRIMACYT and request a Quote by preformatted Email.

    .


    Download Lot Information





    Product Code BHCS - Cryopreserved Beagle Hepatocytes in Suspension - Lot and Order Information
    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the standard thawing protocol.
    • To print lot information, please select print icon at the end of this list.
    Product Code / LOTGenderInventoryViability %Total cells x10exp6 /vialAfter Thawing Viability %After Thawing Recovery x10exp6 /vialTotal number of cells available x10exp6Viability (%) after shaking for 1h at 37 deg CPrice EUR/Vial
    BHCS/BH120208-2aM148210704,157,455129,00
    BHCS/BH120208-2bM178210725,085,057129,00
    BHCS/BH120209-1aF178910783,559,56164,50
    BHCS/BH120209-1bF138910745,672,872129,00
    BHCS/BH120308-aF178410775,898,667129,00
    BHCS/BH120308-bF178410775,491,875129,00
    BHCS/BH121115F188810803,359,47164,50
    BHCS/BH130605F639110756,3396,966193,50
    BHCS/BH130624M329410743,5112,07664,50
    BHCS/BH130820M298410703,498,66064,50
    BHCS/BH130821M288610655,2145,666129,00
    BHCS/BH140129M118916674,953,967129,00
    BHCS/BH140313F218010667,2151,258193,50
    BHCS/BH150702F478510755,3249,176129,00
    BHCS/BH160818F159010796,9103,582193,50
    BHCS/BH170907-1F98810809,383,770193,50
    Q
    This table is sortable by column. Click on the white arrows starts sorting the desired field. Click on the top left arrow resets data to original state.

    Product Code BHCS
    Price per Vial is determined by the number of cells according to the table below:
    2 - 3.9 Mio - EUR 64,50
    4 - 5.9 Mio - EUR 129,00
    6 - 9.9 Mio - EUR 193,50

    Quantity:


    Get in Dialog with PRIMACYT and request a Quote by preformatted Email.
    .



        GSN-185755-I