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Cryopreserved Beagle Dog Hepatocytes from PRIMACYT.

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    Overview
    The liver fulfills many vital processes in mammals. It is the central organ of energy metabolism, responsible for the maintenance of the blood sugar level and the synthesis of plasma proteins under physiological and pathophysiological conditions.

    Hepatocytes are the most prominent cells within the liver. Hepatocytes eliminate toxic substances from the blood. In this biotransformation process transporter proteins (influx and efflux transporter), phase I reactions (cytochrome P450 proteins), phase II reactions (mainly glucuronidation and sulfatation) play a central role.

    Cryopreserved primary hepatocytes are perfectly suited for in vitro metabolism and toxicity / detoxification studies prior to preclinical or clinical tests.

    Dog hepatocytes are isolated from livers obtained from male or female Beagle and are cryopreserved directly after isolation. Donor demographics stating vaccination status and health status of the animals are available for each animal. Cryopreserved Beagle hepatocytes are used in suspension assays and plated in 2D and 3D cell cultures.
    Our Competencies
    PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading company in the field of research of human and animal hepatocytes. We offer fresh and cryopreserved animal hepatocytes in suspension and plated.

    PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation.

    Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.
    Literature
    In drug development, at least two in vivo repeated-dose toxicity studies of a drug candidate have to be performed in two different animal species before human studies can be initiated (https://www.fda.gov/downloads/drugs/guidancecomplianceregulatoryinformation/guidances/ucm292340.pdf). In most cases these species are mouse or rat and dog or monkey.

    Hepatotoxicity is a major cause for failure in drug development. For interpretation of hepatotoxicity data, it is important to understand potential interspecies differences in mechanisms that lead to drug toxicity. This includes
    - drug uptake into the liver,
    - biotransformation of the drug (Phase I and Phase II), and
    - drug efflux from the liver

    Interspecies differences in expression and activities in transport proteins and biotransformation enzymes may result in different intra- and extrahepatic concentrations of the drug.

    Quantification of drug transporter protein by LC/MS has revealed significant interspecies differences in the expression of uptake and efflux transporters. On the other hand, in beagle hepatocytes expression of the two most important Organic Anion Transporters Oatp1a2 and Oatp1b4 (equivalent to human OATP1B1 and OATP1B3) was maintained at the same level as in dog liver tissue (Wang et al. 2014).

    Wang L, Prasad B, Salphati L, Chu X, Gupta A, Hop C E.C.A., Evers R, and Unadkat J. D.: Interspecies Variability in Expression of Hepatobiliary Transporters across Human, Dog, Monkey, and Rat as Determined by Quantitative Proteomics, Drug Metab. Dispos., 2015, 43:367–374
    Our offering
    Primacyt offers certified pools of cryopreserved Beagle hepatocytes and hepatocytes from single donors. Pooled hepatocytes are obtained from 3 individual animal liver perfusions performed in parallel. After quality control, a pool consisting of equal numbers of viable liver cells from each donor is cryopreserved. In addition, hepatocytes from individual donors are cryopreserved for reference purposes.

    Each pool and the individual single donors are comprehensively tested for viability, plate-ability, Cytochrome P450/Phase I and Phase II activities to ensure that all donors are represented equally within the pool.

    Cryopreserved hepatocytes from individual donors are also tested for drug transporter activities.


    Additional Product Info
    This info refers to the product data below:

    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the thawing protocol for hepatocytes.
    • To print lot information, please select the print icon at the end of the lists below. For research use only.
    • Not intended for human or animal diagnostic or therapeutic use.
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    This info refers to the product data below:




    PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation.
    Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.

    Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.



    PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading company in the field of research of human and animal hepatocytes. We offer fresh and cryopreserved animal hepatocytes in suspension and plated.
    The liver fulfills many vital processes in mammals. It is the central organ of energy metabolism, responsible for the maintenance of the blood sugar level and the synthesis of plasma proteins under physiological and pathophysiological conditions.

    Hepatocytes are the most prominent cells within the liver. Hepatocytes eliminate toxic substances from the blood. In this biotransformation process transporter proteins (influx and efflux transporter), phase I reactions (cytochrome P450 proteins), phase II reactions (mainly glucuronidation and sulfatation) play a central role.

    Cryopreserved primary hepatocytes are perfectly suited for in vitro metabolism and toxicity / detoxification studies prior to preclinical or clinical tests.

    Dog hepatocytes are isolated from livers obtained from male or female Beagle and are cryopreserved directly after isolation. Donor demographics stating vaccination status and health status of the animals are available for each animal. Cryopreserved Beagle hepatocytes are used in suspension assays and plated in 2D and 3D cell cultures.
    The liver fulfills many vital processes in mammals. It is the central organ of energy metabolism, responsible for the maintenance of the blood sugar level and the synthesis of plasma proteins under physiological and pathophysiological conditions.

    Hepatocytes are the most prominent cells within the liver. Hepatocytes eliminate toxic substances from the blood. In this biotransformation process transporter proteins (influx and efflux transporter), phase I reactions (cytochrome P450 proteins), phase II reactions (mainly glucuronidation and sulfatation) play a central role.

    Cryopreserved primary hepatocytes are perfectly suited for in vitro metabolism and toxicity / detoxification studies prior to preclinical or clinical tests.

    Dog hepatocytes are isolated from livers obtained from male or female Beagle and are cryopreserved directly after isolation. Donor demographics stating vaccination status and health status of the animals are available for each animal. Cryopreserved Beagle hepatocytes are used in suspension assays and plated in 2D and 3D cell cultures.




    Product Code BHCP-I-Pool. Cryopreserved Platable Beagle Hepatocytes - Certified Pool - Order Information.

    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the standard thawing protocol.
    • To print lot information, please select print icon at the end of this list.

    -190516

    Product Code

    Lot

    Gender

    Inventory (Number of vials)

    Viability %

    Recovery (Number of viable cells/vial)

    Total Number of Cells available

    CYP1A1/1A2 induction x-fold

    EUR per Vial

    BHCP-I-Pool

    BH-170718-4

    Male

    74

    86.0 +/- 3.6

    > 6 Mio

    > 444 Mio

    17.0

    1067,00

    Q

    Product Code BHCP-I-Pool
    Price per Vial is determined by the number of cells according to the table below:
    4 - 5.9 Mio - EUR 711,00
    6 - 9.9 Mio - EUR 1.067

    Quantity:


    Get in Dialog with PRIMACYT and request a Quote by preformatted Email.

    .



    Download Lot Information



    Product Code BHCP-I-T. Cryopreserved Platable Beagle Hepatocytes for Use in Induction and Transporter Studies - Order Information.
    Information about CYP - Activities and Uptake Transporter Activities are contained in the Lot-Info data, if available.


    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the standard thawing protocol.
    • To print lot information, please select print icon at the end of this list.

    -190516

    Product Code

    Lot

    Gender

    Inventory (Number of vials)

    Viability %

    Recovery (Number of viable cells/vial)

    Total Number of Cells available

    CYP1A1/1A2 induction x-fold

    EUR per Vial

    BHCP-I-T

    BH130510-1-1

    Male

    1

    71.8 +/- 2.6

    > 6 Mio

    > 6 Mio

    23.4

    457,00

    BHCP-I-T

    BH130510-1-2

    Male

    10

    72.9 +/- 3.8

    > 6 Mio

    > 60 Mio

    42.5

    457,00

    BHCP-I-T

    BH130802

    Male

    25

    75.3 +/- 10.0

    > 6 Mio

    > 150 Mio

    3.6

    457,00

    BHCP-I-T

    BH140616-1

    Male

    5

    80.8 +/- 6.7

    > 4 Mio

    > 20 Mio

    19.8

    304,50

    BHCP-I-T

    BH140616-2

    Male

    4

    74.4 +/- 11.7

    > 6 Mio

    > 24 Mio

    19.6

    457,00

    Q

    -190301


    Product Code BHCP-I-T
    Price per Vial is determined by the number of cells according to the table below:
    2 - 3.9 Mio - EUR 153,00
    4 - 5.9 Mio - EUR 304,50
    6 - 9.9 Mio - EUR 457,00

    Quantity:


    Get in Dialog with PRIMACYT and request a Quote by preformatted Email.

    .


    Download Lot Information






    Product Code BHCP-I. Cryopreserved Platable Beagle Hepatocytes for Use in Induction Studies - Order Information.
    Information about CYP - Activities are contained in the Lot-Info data, if available.
    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the standard thawing protocol.
    • To print lot information, please select print icon at the end of this list.

    -190516

    Product Code

    Lot

    Gender

    Inventory (Number of vials)

    Viability %

    Recovery (Number of viable cells/vial)

    Total Number of Cells available

    CYP1A1/1A2 induction x-fold

    EUR per Vial

    BHCP-I

    BH150917

    Male

    8

    82.2 +/- 6.1

    > 6 Mio

    > 48 Mio

    22.1

    356,00

    BHCP-I

    BH170907-2

    Female

    10

    80.7 +/- 0.0

    > 6 Mio

    > 60 Mio

    29.5

    356,00

    BHCP-I

    BH180321-1

    Female

    41

    84.4 +/- 7.9

    > 6 Mio

    > 126 Mio

    40.7

    356,00

    BHCP-I

    BH180417-1

    Male

    12

    84.9 +/- 7.9

    > 6 Mio

    > 72 Mio

    46.7

    356,00

    BHCP-I

    BH180417-2

    Male

    12

    93.2 +/- 0.0

    > 4 Mio

    > 48 Mio

    26.8

    237,50

    Q

    Product Code BHCP-I
    Price per Vial is determined by the number of cells according to the table below:
    2 - 3.9 Mio - EUR 119,00
    4 - 5.9 Mio - EUR 237,50
    6 - 9.9 Mio - EUR 356.00

    Quantity:


    Get in Dialog with PRIMACYT and request a Quote by preformatted Email.

    .


    Download Lot Information





    Product Code BHCS - Cryopreserved Beagle Hepatocytes in Suspension - Lot and Order Information
    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the standard thawing protocol.
    • To print lot information, please select print icon at the end of this list.

    -190516

    Product Code

    Lot

    Gender

    Inventory (Number of vials)

    Viability %

    Recovery (Number of viable cells/vial)

    Total Number of Cells available

    Viability (%) after shaking for 1-2 h at 37°C

    EUR per Vial

    BHCS

    BH121115

    Female

    18

    85.8 +/- 7.8

    > 2 Mio

    > 36 Mio

    pending

    68,00

    BHCS

    BH130605

    Female

    63

    73.7 +/- 12.3

    > 6 Mio

    > 378 Mio

    66

    203,00

    BHCS

    BH130624

    Male

    32

    74.6 +/- 10.9

    > 2 Mio

    > 64Mio

    76

    68,00

    BHCS

    BH150211-4

    Male

    19

    73.3 +/- 7.6

    > 6 Mio

    > 114 Mio

    66

    203,00

    BHCS

    BH151112

    Male

    34

    74,0 +/- 6,5

    > 6 Mio

    > 204 Mio

    70

    203,00

    Q

    Product Code BHCS
    Price per Vial is determined by the number of cells according to the table below:
    2 - 3.9 Mio - EUR 68,00
    4 - 5.9 Mio - EUR 135,50
    6 - 9.9 Mio - EUR 203,00

    Quantity:


    Get in Dialog with PRIMACYT and request a Quote by preformatted Email.
    .



        GSN-185755-I