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Fresh Göttingen Minipig Hepatocytes from PRIMACYT

Our fresh Göttingen Minipig hepatocytes are of highest quality. The level of quality insurance is identical with human hepatocytes. Typically we offer highest viability and functionality levels. In combination with appropriate cell morphology, our cell technology is one instrument for adequate in vitro - in vivo correlations for your purposes.
Characterization methods of fresh animal hepatocytes include the monitoring of liver specific functions, activities of drug transporters and the induction of cytochrome P450 activities. We also monitor and document cell morphology, attachment efficiency, monolayer confluency for platable cells and viability stability over time.

Göttingen Minipig hepatocytes are available in suspension and in multi-well plates (6-, 12-, 24- and 96-well plate). Our Primary Animal hepatocytes are available in suspension. Minimum order is 10 million viable cells. Suspension cells are for immediate use after arrival.




Fresh Göttingen Minipig Hepatocytes in Suspension

Products in Suspension
Suspensions will be sent on crushed ice on the day of isolation and should be used on the day of receipt. Although hepatocytes in suspension are normally plateable within 20-24 hours after isolation, we do not guarantee the plateability of these cells, and recommend to order plated hepatocytes if this is the intended use. Included with each suspension order are MSDS and donor demographics when applicable (human, Cynomolgus, Beagle), including serology data and vaccinations (Cynomolgus, Beagle).

Biohazard warning: Tissue fractions such as hepatocytes should be considered as potentially biohazardous. In case of an incident and hazardous circumstances , they should be treated as biohazards in the laboratory.
Only for research purposes. Not for use in human diagnostics or therapeutics.


Minimum Order 30 M Cells

Product No.
Product
Specification
Packaging
EUR per 1 Mio Cells

GMHS-01

In suspension

> 30 Mio viable cells

Vial

62,00

GMHS-01

In suspension

> 100 Mio viable cells

Vial

56,00

GMHS-01

In suspension

> 200 Mio viable cells

Vial

49,50


Quantity:






Fresh Minipig Hepatocytes Plated

Plated Products
Plated products will be sent at ambient temperature on day 1 after isolation and should be cultured at 37°C with 95% humidity and 5% CO2 atmosphere. Included with each plated order are following documents: datasheet (with microscopic images of cells prior to shipping), manual for culture of primary hepatocytes, MSDS, and donor demographics when applicable (human, Cynomolgus, Beagle), including serology data and vaccinations (Cynomolgus, Beagle).

Cells in plates require light protected culture at 37 ℃ and 5 % CO2 . However, conditions may be adjusted according to the body temperature of the individual species and with regard to the individual experimental design.

It is recommended to culture mammal hepatocytes on collagen coated culture plates in HHMM (Human Hepatocyte Maintenance Medium), a serum-free culture medium containing Hepatocyte Growth Factor and Epidermal Growth Factor. Rat hepatocytes may also be cultured in HGM (Hepatocyte Growth Medium) instead of HHMM. For mouse hepatocytes we recommend the use of MHM (Mouse Hepatocyte Medium).

Biohazard warning: Tissue fractions such as hepatocytes should be considered as potentially biohazardous. In case of an incident and hazardous circumstances , they should be treated as biohazards in the laboratory.
Only for research purposes. Not for use in human diagnostics or therapeutics.

Product No.
Product
Specification
Packaging
EUR per Plate

GMHP-06

6 well plate
collagen coated

1.2 Mio cells per well

Minimum - 2 plates

please ask

GMHP-06

6 well plate
collagen coated

1.2 Mio cells per well

3-5 plates

490,00

GMHP-06

6 well plate
collagen coated

1.2 Mio cells per well

6-10 plates

410,00

GMHP-06

6 well plate
collagen coated

1.2 Mio cells per well

> 10 plates

380,00

Product No.
Product
Specification
Packaging
EUR per Plate

GMHP-12

12 well plate
collagen coated

600.000 cells per well

Minimum - 2 plates

please ask

GMHP-12

12 well plate
collagen coated

600.000 cells per well

3-5 plates

490,00

GMHP-12

12 well plate
collagen coated

600.000 cells per well

6-10 plates

410,00

GMHP-12

12 well plate
collagen coated

600.000 cells per well

> 10 plates

380,00

Quantity:




Product No.
Product
Specification
Packaging
EUR per Plate

GMHP-24

24 well plate
collagen coated

300.000 cells per well

Minimum - 2 plates

please ask

GMHP-24

24 well plate
collagen coated

300.000 cells per well

3-5 plates

490,00

GMHP-24

24 well plate
collagen coated

300.000 cells per well

6-10 plates

410,00

GMHP-24

24 well plate
collagen coated

300.000 cells per well

> 10 plates

380,00

Product No.
Product
Specification
Packaging
EUR per Plate

GMHP-96

96 well plate
collagen coated

50.000 cells per well

Minimum - 2 plates

please ask

GMHP-96

96 well plate
collagen coated

50.000 cells per well

3-5 plates

520.00

GMHP-96

96 well plate
collagen coated

50.000 cells per well

6-10 plates

440.00

GMHP-96

96 well plate
collagen coated

50.000 cells per well

> 10 plates

405.00

Quantity:


More Information

Fig 1: Göttingen Minipig hepatocytes at day 3 of culture in 24well plates cultured with HHMM (left: fresh Minipig hepatocytes, right: cryopreserved Minipig hepatocytes)..

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Hepatocytes are the most prominent cells of the liver. They synthesize albumin, produce urea and eliminate toxic substances from the blood. In this biotransformation process transporter proteins (influx and efflux transporter) and phase I+II reactions play a central role. Primary hepatocytes are perfectly suited for in vitro metabolism and toxicity studies prior to preclinical tests. Minipig hepatocytes are isolated from livers obtained from male or female Göttingen Minipigs and are available fresh in suspensions or in various culture formats (6, 12, 24 and 96well) as well as cryopreserved cells. We recommend to culture Minipig hepatocytes on collagen coated culture plates in PRIMACYT’s serum-free HHMM (Human Hepatocyte Maintenance Medium). Hepatocyte specific morphology and cytochrome P450 protein activities are maintained and/or remain inducible under these culture conditions (Fig. 1 and 2).


Fig 2: Induction of Ethoxyresorufin-O-deethylase (EROD) activity by ß-Naphthoflavone (ß-NF) for 48 h in Göttingen Minipig hepatocytes cultured with HHMM (left: fresh hepatocytes, induction day 3-5; right: cryopreserved hepatocytes, induction day 1-3 and day 5-7).
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Final Notice:
All personnel involved in potentially biologically hazardous activity share biosafety responsibility and must follow specified procedures, take appropriate training, act responsibly, and report incidents and hazardous circumstances. They should inform their supervisor of any personal condition such as illness, medications, pregnancy, or reduced immunity, which could make their work more hazardous to themselves and others.