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Fresh Primary Human Hepatocytes from PRIMACYT

Primary human liver cells are prepared for in-vitro cultures. The individual donor based cells are best suited to demonstrate major tissue functions, such as ammonia detoxification or drug metabolism. Isolated human hepatocytes act as a living biological system. They maintain hepatocyte morphology and function over long time. We believe, that our HEPAC2 model is very close to in vivo conditions, thus representing the gold standard in hepatocyte research.

Validate your research on the basis of transformed cell lines or animal models by using fresh human hepatocytes.

PRIMACYT is specialized on its animal serum free HEPAC2 cell culture system and is a profound supplier of fresh hepatocytes. PRIMACYT has a long track record and experience in hepatocyte isolation and hepatocyte culture.
Primary human liver cells are prepared for in-vitro cultures. The individual donor based cells are best suited to demonstrate major tissue functions, such as ammonia detoxification or drug metabolism. Isolated human hepatocytes act as a living biological system. They maintain hepatocyte morphology and function over long time.

We believe, that the described HEPAC2 model is very close to in vivo conditions, thus representing the gold standard in hepatocyte research.

Application Fields

Short term studies of plated hepatocytes are cultures up to 7 days. Use of HM is recommended. Culture medium HHMM should be used for long term studies (> 7 days).
  • Studies of cell metabolism- metabolic pathway identification
    - metabolic profiling
    - metabolism of stable compounds
    - determination of metabolic routes in metabolite formation
    - assessment of effects on hepatocellular functions
    - assessment of protection against cancer (reactive oxygen species)
  • Drug development studies- drug-drug interaction
    - evaluation of drug metabolism
    - drug-mediated responses
  • Toxicity studies- hepatotoxicity, detoxification effects
    - uptake and efflux transporter activities
  • Studies on enzyme activities- assessment of potential enzyme induction or inhibition
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Features / Benefits

  • Human tissue model research prior to clinical testing
  • Ready-to-use / organized culturesFresh hepatocytes come ready to use on collagen coated plates.
  • Multiple formats for your convenienceAvailable in suspension for short term studies or plated in 6/12/24/96 well plates or in flasks (25/75 sqcm). Other formats on request.
  • Short term delivery possibilitiesPRIMACYT is permanently updating the delivery schedule via the email alert system and on the on the homepage.
  • Fast Track Processing for our registered customers.
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Fresh Human Hepatocytes in Suspension

Our Primary Human hepatocytes are available in suspension. Minimum order is 10 million viable cells). Suspension cells are for immediate use after arrival. .

Products in Suspension

Suspensions will be sent on crushed ice on the day of isolation and should be used on the day of receipt. Included with each suspension order are following documents: Animal demographics (Rat) as datasheet, instructions for recovery of cells. Although hepatocytes in suspension are normally platable within 20-24 hours after isolation, we do not guarantee the plateability of these cells, and recommend to order plated hepatocytes if this is the intended use.

Biohazard warning: Tissue fractions such as hepatocytes should be considered as potentially biohazardous, and should be treated as biohazards in the laboratory. Only for research purposes. Not for use in human diagnostics or therapeutics.

Minimum Order 30 M Cells, setup fee may apply.

Product No.
Product
Specification
Packaging
EUR per 1 Mio Cells

HHS-01

in suspension

> 10 Mio viable cells

Vial

68,00

HHS-01

in suspension

> 100 Mio viable cells

Vial

61,20

Quantity:




Fresh Human Hepatocytes Plated

Plated Products
Plated products will be sent at ambient temperature on day 1 after isolation and should be stored at 37°C with 95% humidity and 5% CO2 atmosphere. Included with each plated order are following documents: Animal Demographics (Rat), datasheet (with microscopic images of cells prior to shipping), manual for culture of primary hepatocytes.

Cells in plates require light protected storage at 37 ℃ and 5 % CO2 .

It is recommended to culture Cynomolgus hepatocytes on collagen coated culture plates in HHMM (Human Hepatocyte Maintenance Medium), a serum-free culture medium containing Hepatocyte Growth Factor and Epidermal Growth Factor.

Biohazard warning: Tissue fractions such as hepatocytes should be considered as potentially biohazardous, and should be treated as biohazards in the laboratory. Only for research purposes. Not for use in human diagnostics or therapeutics.
Product No.
Product
Specification
Packaging
EUR per Plate

HHP-06

6 well plate
collagen coated

1.2 Mio cells per well

1-2 plates

685,00

HHP-06

6 well plate
collagen coated

1.2 Mio cells per well

3-5 plates

685,00

HHP-06

6 well plate
collagen coated

1.2 Mio cells per well

6-10 plates

620,00

HHP-06

6 well plate
collagen coated

1.2 Mio cells per well

> 10 plates

580,00

Product No.
Product
Specification
Packaging
EUR per Plate

HHP-12

12 well plate
collagen coated

600.000 cells per well

1-2 plates

685,00

HHP-12

12 well plate
collagen coated

600.000 cells per well

3-5 plates

685,00

HHP-12

12 well plate
collagen coated

600.000 cells per well

6-10 plates

620,00

HHP-12

12 well plate
collagen coated

600.000 cells per well

> 10 plates

580,00

Quantity:



Product No.
Product
Specification
Packaging
EUR per Plate

HHP-24

24 well plate
collagen coated

300.000 cells per well

1-2 plates

685,00

HHP-24

24 well plate
collagen coated

300.000 cells per well

3-5 plates

685,00

HHP-24

24 well plate
collagen coated

300.000 cells per well

6-10 plates

620,00

HHP-24

24 well plate
collagen coated

300.000 cells per well

> 10 plates

580,00

Product No.
Product
Specification
Packaging
EUR per Plate

HHP-96

96 well plate
collagen coated

50.000 cells per well

1-2 plates

685.00

HHP-96

96 well plate
collagen coated

50.000 cells per well

3-5 plates

685.00

HHP-96

96 well plate
collagen coated

50.000 cells per well

6-10 plates

620.00

HHP-96

96 well plate
collagen coated

50.000 cells per well

> 10 plates

580.00

Quantity:


Biohazard warning: Tissue fractions such as hepatocytes should be considered as potentially biohazardous, and should be treated as biohazards in the laboratory. Only for research purposes. Not for use in human diagnostics or therapeutics.