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Fresh Beagle (Dog) Hepatocytes from PRIMACYT

Beagle hepatocytes can be made available in suspension and in multi-well plates (6-, 12-, 24- and 96-well plate) on request. Please ask for availability or custom-made preparations.
Our fresh Beagle Dog hepatocytes are of the highest quality. The level of quality insurance is identical to human hepatocytes. Typically we offer the highest viability and functionality levels. In combination with appropriate cell morphology, our cell technology is one instrument for adequate in vitro - in vivo correlations for your purposes.

Characterization methods of fresh animal hepatocytes include monitoring liver-specific functions, activities of drug transporters, and the induction of cytochrome P450 activities. We also monitor and document cell morphology, attachment efficiency, monolayer confluency for plateable cells, and the overall appearance.
Our fresh Beagle Dog hepatocytes are of the highest quality. The level of quality insurance is identical to human hepatocytes. Typically we offer the highest viability and functionality levels. In combination with appropriate cell morphology, our cell technology is one instrument for adequate in vitro - in vivo correlations for your purposes.
Characterization methods of fresh animal hepatocytes include monitoring liver-specific functions, activities of drug transporters, and the induction of cytochrome P450 activities. We also monitor and document cell morphology, attachment efficiency, monolayer confluency for plate-able cells, and the overall appearance.

Fresh Beagle Hepatocytes in Suspension

  • Products in SuspensionSuspensions will be sent on crushed ice on the day of isolation and should be used on receipt. Included with each suspension order are the following documents: Animal demographics as a datasheet, instructions for recovery of cells. Although hepatocytes in suspension are typically plateable within 20-24 hours after isolation, we do not guarantee these cells and recommend ordering plated hepatocytes if this is the intended use.
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Fresh Beagle Dog Hepatocytes Plated

  • Plated ProductsPlated products will be dispatched at ambient temperature on day one after isolation and stored at 37°C with 95% humidity and 5% CO2 atmosphere. Included with each order are the following documents: Animal Demographics, datasheet (with microscopic images of cells before shipping), manual for primary hepatocytes' culture.

    Cells in plates require light-protected storage at 37°C and 5 % CO2.
    It is recommended to culture Beagle Dog hepatocytes on collagen-coated culture plates in HHMM (Human Hepatocyte Maintenance Medium), a serum-free culture medium containing Hepatocyte Growth Factor and Epidermal Growth Factor.
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Biohazard warning: Tissue fractions such as hepatocytes should be considered as potentially biohazardous, and should be treated as biohazards in the laboratory. Only for research purposes. Not for use in human diagnostics or therapeutics.


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Fig 1: Beagle hepatocytes at day 1 of culture stained with goat anti dog albumin (green fluorescence, upper panel) and urea release (lower panel) from day 2 to 23 in dog hepatocytes cultured with HHMM.

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Cell specification


The liver fulfills many vital processes in mammals. It is the central organ of energy metabolism (glycolysis, gluconeogenesis, lipid metabolism, amino acid metabolism, and ureagenesis). It is responsible for maintaining the blood sugar level and the synthesis of plasma proteins under physiological and pathophysiological conditions. Hepatocytes are the most prominent cells within the liver. Hepatocytes eliminate toxic substances from the blood. In this biotransformation process, transporter proteins (influx and efflux transporter), phase I reactions (cytochrome P450 proteins), phase II reactions (mainly glucuronidation and sulfation) play a central role. Primary hepatocytes are ideal for in vitro metabolism and toxicity/detoxification studies before preclinical or clinical tests. Propagation of hepatocytes for cell transplantation, three-dimensional culture systems, and culture in bioartificial liver support devices is now under investigation.

Dog hepatocytes are isolated from livers obtained from male or female Beagles and are available fresh as suspensions or in various culture formats (6, 12, 24, and 96 well plates). Special configurations are available on request. Donor demographics stating vaccination status and health status of the animals are available for each animal.

Recommended medium and culture conditions


It is recommended to culture Beagle hepatocytes on collagen-coated culture plates in HHMM (Human Hepatocyte Maintenance Medium), a serum-free culture medium containing Hepatocyte Growth Factor and Epidermal Growth Factor. Hepatocyte-specific morphology and functions like albumin and urea synthesis and cytochrome P450 protein activities are maintained and remain inducible under these culture conditions for several weeks (Fig. 1 and 2). For short-term cultures of 7 days or less, Hepatocyte Medium (HM) may also be used as an economically priced medium.

Fig 2: Induction of Ethoxyresorufin-O-deethylase (EROD) activity by ß-Naphthoflavone (ß-NF) in Beagle hepatocytes cultured with HHMM. Control = hepatocytes cultured for 48 hours in the absence of ß-NF. 25 µM ß-NF = hepatocytes cultured for 48 hours in the presence of 25 µM ß-NF. 50 µM ß-NF = hepatocytes cultured for 48 hours in the presence of 50 µM ß-NF.
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More Information

Fig 1: Beagle hepatocytes at day 1 of culture stained with goat anti dog albumin (green fluorescence, upper panel) and urea release (lower panel) from day 2 to 23 in dog hepatocytes cultured with HHMM.

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Cell specification


The liver fulfills many vital processes in mammals. It is the central organ of energy metabolism (glycolysis, gluconeogenesis, lipid metabolism, amino acid metabolism, and ureagenesis). It is responsible for maintaining the blood sugar level and the synthesis of plasma proteins under physiological and pathophysiological conditions. Hepatocytes are the most prominent cells within the liver. Hepatocytes eliminate toxic substances from the blood. In this biotransformation process, transporter proteins (influx and efflux transporter), phase I reactions (cytochrome P450 proteins), phase II reactions (mainly glucuronidation and sulfation) play a central role. Primary hepatocytes are ideal for in vitro metabolism and toxicity/detoxification studies before preclinical or clinical tests. Propagation of hepatocytes for cell transplantation, three-dimensional culture systems, and culture in bioartificial liver support devices is now under investigation.

Dog hepatocytes are isolated from livers obtained from male or female Beagles and are available fresh as suspensions or in various culture formats (6, 12, 24, and 96 well plates). Special configurations are available on request. Donor demographics stating vaccination status and health status of the animals are available for each animal.

Recommended medium and culture conditions


It is recommended to culture Beagle hepatocytes on collagen-coated culture plates in HHMM (Human Hepatocyte Maintenance Medium), a serum-free culture medium containing Hepatocyte Growth Factor and Epidermal Growth Factor. Hepatocyte-specific morphology and functions like albumin and urea synthesis and cytochrome P450 protein activities are maintained and remain inducible under these culture conditions for several weeks (Fig. 1 and 2). For short-term cultures of 7 days or less, Hepatocyte Medium (HM) may also be used as an economically priced medium.

Fig 2: Induction of Ethoxyresorufin-O-deethylase (EROD) activity by ß-Naphthoflavone (ß-NF) in Beagle hepatocytes cultured with HHMM. Control = hepatocytes cultured for 48 hours in the absence of ß-NF. 25 µM ß-NF = hepatocytes cultured for 48 hours in the presence of 25 µM ß-NF. 50 µM ß-NF = hepatocytes cultured for 48 hours in the presence of 50 µM ß-NF.
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You have arrived on this page because you are generally interested in fresh primary animal hepatocytes, hepatocytes in suspension, plated hepatocytes, beagle dog hepatocytes and in cryopreserved hepatocytes. Should you need advice and information about topic areas such as monolayer confluency, enzyme activities, drug metabolizing, attachment efficiency, cell morphology, viability stability or in enzyme concentration, response compensation, and liability, or generally in biochemical reactions related to beagle dog hepatocytes, please contact us.