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Cryopreserved Rainbow Trout (Oncorhynchus mykiss) Hepatocytes from PRIMACYT.

Pooled Fish Hepatocytes - a new in-vitro model metabolic System.

PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading Company in the field of research of human and animal hepatocytes. We offer fresh and cryopreserved animal hepatocytes in suspension and plated.

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    Overview
    Animals like Rainbow trout (Oncorhynchus mykiss) have been used in vivo by researchers for decades to assess bioaccumulation, metabolism, and environmental risk of chemicals, including drugs, xenobiotics, cosmetics, fragrances, flavoring substances, herbicides and crop protection products. Apart from Zebrafish, Rainbow trout is the best-studied fish species.

    The OECD Test Guideline 319A Determination of in vitro intrinsic clearance using cryopreserved rainbow trout hepatocytes (RT-HEP) was released on June 25, 2018. Primacyt now offers cryopreserved pools of rainbow trout hepatocytes prepared and characterized in accordance with this guideline.
    Our Competencies
    PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation.

    Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.
    Rainbow Trout - a new in-vitro System
    More recently, researchers of the US Environmental Protection Agency (EPA), DuPont Haskell, the University of Bern and Fraunhofer IME have introduced trout hepatocytes as a reliable in-vitro system for bioaccumulation and clearances studies.

    Measurements of biotransformation rates in primary fish hepatocytes can be scaled up to the whole animal and thereby can provide data used in computational models to predict chemical bio-concentration factors in animals (K.A. Faye et al. 2014, I. Bischof et al. 2016).

    These and other supporting research results lead to the implementation of OECD Test Guidelines 319A and 319B on the determination of intrinsic clearance using cryopreserved rainbow trout hepatocytes and liver S9 subcellular fractions.

    Access the webpage for this guideline and download the document:



    Literature
    Tust M., Kohler M., Lagojda A, and Lamshoeft M: Comparison of the in vitro assays to investigate the hepatic metabolism of seven pesticides in Cyprinus carpio and Oncorhynchus mykiss.
    https://doi.org/10.1016/j.chemosphere.2021.130254
    Access the publication

    Intra- and interlaboratory reliability of a cryopreserved trout hepatocyte assay for the prediction of chemical bioaccumulation potential.
    Access the publication

    Hepatocytes as in vitro test system to investigate metabolite patterns of pesticides in farmed rainbow trout and common carp: Comparison between in vivo and in vitro and across species.
    Access the publication

    Reliability of In Vitro Methods used to Measure Intrinsic Clearance of Hydrophobic Organic Chemicals by Rainbow Trout: Results of an International Ring Trial.
    Access the Publication

    Our Offering
    In accordance with OECD guideline 319A Primacyt now offers cryopreserved pools of primary hepatocytes from rainbow trout kept under controlled conditions at Fraunhofer Institute for Marine Biotechnology and Cell Technology for at least two weeks in continuous flow water. Each lot is comprehensively tested for high viability and Cytochrome P450/Phase I and Phase II activities.
    Additional Product Info
    This info refers to the product data below:
    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the thawing protocol for fish hepatocytes.
    • To print lot information, please select the print icon at the end of the lists belowFor research use only.
    • Not intended for human or animal diagnostic or therapeutic use.
    Contact us now
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    Download Area (MSDS, Manuals)
    Please click on the buttons below to download MSDS data and the Plating and Thawing Manual.






    Animals like Rainbow trout (Oncorhynchus mykiss) have been used in vivo by researchers for decades to assess bioaccumulation, metabolism, and environmental risk of chemicals, including drugs, xenobiotics, cosmetics, fragrances, flavoring substances, herbicides and crop protection products. Apart from Zebrafish, Rainbow trout is the best-studied fish species.

    More recently, researchers of the US Environmental Protection Agency (EPA), DuPont Haskell, the University of Bern and Fraunhofer IME have introduced trout hepatocytes as a reliable in-vitro system for bioaccumulation and clearances studies. Measurements of biotransformation rates in primary fish hepatocytes can be scaled up to the whole animal and thereby can provide data used in computational models to predict chemical bio-concentration factors in animals (K.A. Faye et al. 2014, I. Bischof et al. 2016). These and other supporting research results lead to the draft of an OECD Test Guideline on the determination of intrinsic clearance using cryopreserved rainbow trout hepatocytes and liver S9 subcellular fractions.

    Primacyt now offers cryopreserved pools of primary hepatocytes from rainbow trout kept under controlled conditions at Fraunhofer Institute for Marine Biotechnology and Cell Technology for at least two weeks in continuous flow water. Each lot is comprehensively tested for high viability and Cytochrome P450/Phase I and Phase II activities.

    Literature:
    K.A. Faye, R.T. Mingoia, I. Göritz, D.L. Nabb, A.D. Hoffman, B.D. Ferrel, H.M. Peterson, J.W. Nichols, H. Segner, and X. Han: Intra- and Interlaboratory Reliability of a Cryopreserved Trout Hepatocyte Assay for the Prediction of Chemical Bioaccumulation Potential. Environmental Science & Technology 2014, 48, 8170-8178

    I. Bischof, J. Köster, H. Segner, and C. Schlechtriem: Hepatocytes as in vitro test system to investigate to investigate metabolite patterns of pesticides in farmed rainbow trout and common carp: Comparison between in vivo and in vitro and across species. Comparative Biochemistry and Physiology, Part C, 2016, 187, 62-73

    PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation.
    Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.


    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the standard thawing protocol.
    • To print lot information, please select print icon at the end of this list.

    Product Code RTHCS - Rainbow Trout Hepatocytes Cryopreserved for use in Suspension - Pools of sexually immature animals kept under controlled husbandry conditions – Lot and Order Information

    Click on the Lot-Info Hyperlink to see the detailed Lot Information.

    - 2020-01-03

    Product Code

    Lot

    Gender

    Viability after isolation (%)

    Inventory (Number of vials)l

    Viability after Thawing (%)

    Recovery (Viable number of cells/vial)

    Total number of cells available

    Viability (%) after shaking for 2 h at 14°C

    EUR per Vial
    RTHCS

    RTH
    180212
    -1 Pool

    2 male, 1 female

    95

    44

    89.4

    > 2 Mio

    > 88 Mio

    96.4

    388.00
    RTHCS

    RTH
    180212
    -2 Pool

    1 male, 2 female

    96

    7

    96.7

    > 2 Mio

    > 14 Mio

    92.2

    388.00
    RTHCS

    RTH
    180214
    -2 Pool

    2 female

    97

    4

    97.7

    > 4 Mio

    > 16 Mio

    94.4

    534.00
    RTHCS

    RTH
    180219
    -Pool

    6 female

    97

    84

    94.9

    > 2 Mio

    > 168 Mio

    96.7

    388.00
    RTHCS

    RTH
    180221-
    Pool

    2 male, 2 female

    97

    33

    98.0

    > 4 Mio

    > 132 Mio

    97.9

    534.00
    RTHCS

    RTH200415

    2 male, 4 female

    97

    151

    99.5

    > 10 Mio

    > 1.510 Mio

    98.3

    880.00
    Q


    Quantity:

    Download Lot Information




    Product Code FHCS - Cryopreserved trout hepatocytes for use in Suspension - Single donors, animals kept under non-controlled husbandry conditions – Lot and Order Information

    Click on the Lot-Info Hyperlink to see the detailed Lot Information.
    Product Code FHCS

    - 2020-01-03

    Product Code

    Lot

    Gender

    Viability after isolation (%)

    Inventory (Number of vials)
    Viability after Thawing (%)

    Recovery (Viable number of cells/vial)

    Total number of cells available

    Viability (%) after shaking for 2 h at 14°C

    EUR per Vial
    FHCS

    FH160727-4

    M

    96

    7

    98.8

    > 6 Mio

    > 42 Mio

    91.9

    574.00
    FHCS

    FH160907-1

    M

    94

    4

    94.2

    > 2 Mio

    > 8 Mio

    95.2

    287.00
    FHCS

    FH160907-2

    F

    95

    4

    94.4

    > 4 Mio

    > 16 Mio

    92.1

    431.00
    Q

    Quantity:


    Download Lot Information