FP - 2021-0729
Cryopreserved Rainbow Trout (Oncorhynchus mykiss) Hepatocytes from PRIMACYT.
Pooled Fish Hepatocytes - a new in-vitro model metabolic System.
PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading Company in the field of research of human and animal hepatocytes. We offer fresh and cryopreserved animal hepatocytes in suspension and plated.
Animals like Rainbow trout (Oncorhynchus mykiss) have been used in vivo by researchers for decades to assess bioaccumulation, metabolism, and environmental risk of chemicals, including drugs, xenobiotics, cosmetics, fragrances, flavoring substances, herbicides and crop protection products. Apart from Zebrafish, Rainbow trout is the best-studied fish species.
More recently, researchers of the US Environmental Protection Agency (EPA), DuPont Haskell, the University of Bern and Fraunhofer IME have introduced trout hepatocytes as a reliable in-vitro system for bioaccumulation and clearances studies. Measurements of biotransformation rates in primary fish hepatocytes can be scaled up to the whole animal and thereby can provide data used in computational models to predict chemical bio-concentration factors in animals (K.A. Faye et al. 2014, I. Bischof et al. 2016). These and other supporting research results lead to the draft of an OECD Test Guideline on the determination of intrinsic clearance using cryopreserved rainbow trout hepatocytes and liver S9 subcellular fractions.
Primacyt now offers cryopreserved pools of primary hepatocytes from rainbow trout kept under controlled conditions at Fraunhofer Institute for Marine Biotechnology and Cell Technology for at least two weeks in continuous flow water. Each lot is comprehensively tested for high viability and Cytochrome P450/Phase I and Phase II activities.
Literature:
K.A. Faye, R.T. Mingoia, I. Göritz, D.L. Nabb, A.D. Hoffman, B.D. Ferrel, H.M. Peterson, J.W. Nichols, H. Segner, and X. Han: Intra- and Interlaboratory Reliability of a Cryopreserved Trout Hepatocyte Assay for the Prediction of Chemical Bioaccumulation Potential. Environmental Science & Technology 2014, 48, 8170-8178
I. Bischof, J. Köster, H. Segner, and C. Schlechtriem: Hepatocytes as in vitro test system to investigate to investigate metabolite patterns of pesticides in farmed rainbow trout and common carp: Comparison between in vivo and in vitro and across species. Comparative Biochemistry and Physiology, Part C, 2016, 187, 62-73
PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation.
Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.
- Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
- Viability: Denotes post thaw results using the standard thawing protocol.
- To print lot information, please select print icon at the end of this list.
Product Code RTHCS - Rainbow Trout Hepatocytes Cryopreserved for use in Suspension - Pools of sexually immature animals kept under controlled husbandry conditions – Lot and Order Information
Click on the Lot-Info Hyperlink to see the detailed Lot Information.
Product Code |
Lot |
Gender |
Viability after isolation (%) |
Inventory (Number of vials)l |
Viability after Thawing (%) |
Recovery (Viable number of cells/vial) |
Total number of cells available |
Viability (%) after shaking for 2 h at 14°C |
EUR per Vial |
|---|---|---|---|---|---|---|---|---|---|
RTHCS |
RTH |
2 male, 1 female |
95 |
44 |
89.4 |
> 2 Mio |
> 88 Mio |
96.4 |
388.00 |
RTHCS |
RTH |
1 male, 2 female |
96 |
7 |
96.7 |
> 2 Mio |
> 14 Mio |
92.2 |
388.00 |
RTHCS |
RTH |
2 female |
97 |
4 |
97.7 |
> 4 Mio |
> 16 Mio |
94.4 |
534.00 |
RTHCS |
RTH |
6 female |
97 |
87 |
94.9 |
> 2 Mio |
> 174 Mio |
96.7 |
388.00 |
RTHCS |
RTH |
2 male, 2 female |
97 |
36 |
98.0 |
> 4 Mio |
> 144 Mio |
97.9 |
534.00 |
RTHCS |
RTH200415 |
2 male, 4 female |
97 |
151 |
99.5 |
> 10 Mio |
> 1.510 Mio |
98.3 |
880.00 |
Q
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Product Code FHCS - Cryopreserved trout hepatocytes for use in Suspension - Single donors, animals kept under non-controlled husbandry conditions – Lot and Order Information
Click on the Lot-Info Hyperlink to see the detailed Lot Information.
Product Code FHCS
Product Code |
Lot |
Gender |
Viability after isolation (%) |
Inventory (Number of vials) |
Viability after Thawing (%) |
Recovery (Viable number of cells/vial) |
Total number of cells available |
Viability (%) after shaking for 2 h at 14°C |
EUR per Vial |
|---|---|---|---|---|---|---|---|---|---|
FHCS |
FH160727-4 |
M |
96 |
7 |
98.8 |
> 6 Mio |
> 42 Mio |
91.9 |
574.00 |
FHCS |
FH160907-1 |
M |
94 |
4 |
94.2 |
> 2 Mio |
> 8 Mio |
95.2 |
287.00 |
FHCS |
FH160907-2 |
F |
95 |
4 |
94.4 |
> 4 Mio |
> 16 Mio |
92.1 |
431.00 |
Q
Download Lot Information