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Cryopreserved Common Carp (Cyprinus carpio) Hepatocytes.

Pooled Fish Hepatocytes - for new in-vitro model metabolic System.


PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading Company in the field on research of human and animal hepatocytes. We offer fresh and cryopreserved animal hepatocytes in suspension and plated.

PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation.

Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.
PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation.
Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.

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    Overview
    Sweetwater fish like Common Carp (Cyprinus carpio) have been used in vivo by researchers for decades to assess bioaccumulation, metabolism, and environmental risks of chemicals, including herbicides, crop protection products, and fertilizers. Apart from Rainbow trout, Common carp is one of the best-studied fish species.

    The OECD released on June 25, 2018 their Test Guideline 319A, Determination of in vitro intrinsic clearance using cryopreserved rainbow trout . PRIMACYT now offers cryopreserved common carp hepatocytes (pooled or single donor) prepared following this guideline.

    Our Competencies
    PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as a member of the laboratory network responsible for the method validation.

    Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.

    Literature
    Hepatocytes as in vitro test system to investigate metabolite patterns of pesticides in farmed rainbow trout and common carp: Comparison between in vivo and in vitro and across species.

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    Smeets JM, Rankouhi TR, Nichols KM, Komen H, Kaminski NE, Giesy JP, van den Berg M.:
    In vitro vitellogenin production by carp (Cyprinus carpio) hepatocytes as a screening method for determining (anti)estrogenic activity of xenobiotics. Toxicol Appl Pharmacol. 1999 May 15;157(1):68-76.

    … Read more


    Fan Yanhong, He Chenghu, Liu Guofang, Zhang Haibin: Optimization of the isolation and cultivation of Cyprinus carpio primary hepatocytes, Cytotechnology (2008) 58:85–92 DOI 10.1007/s10616-008-9169-5


    … Read more

    Our Offering
    Following the OECD guideline 319A Primacyt now offers cryopreserved primary hepatocytes from commoin carp kept under controlled conditions for at least two weeks in continuous flow water. Each lot is comprehensively tested for high viability and Cytochrome P450/Phase I activities and is suitable for at least 24 hours in suspension assays. For the first time. plateable cryopreserved carp hepatocytes are made available for researchers worldwide.

    Additional Product Info
    This info refers to the product data below:

    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the thawing protocol for fish hepatocytes.
    • To print lot information, please select the print icon at the end of the lists belowFor research use only.
    • Not intended for human or animal diagnostic or therapeutic use.


    Animals like Rainbow trout (Oncorhynchus mykiss) have been used in vivo by researchers for decades to assess bioaccumulation, metabolism, and environmental risk of chemicals, including drugs, xenobiotics, cosmetics, fragrances, flavoring substances, herbicides and crop protection products. Apart from Zebrafish, Rainbow trout is the best-studied fish species.

    More recently, researchers of the US Environmental Protection Agency (EPA), DuPont Haskell, the University of Bern and Fraunhofer IME have introduced trout hepatocytes as a reliable in-vitro system for bioaccumulation and clearances studies. Measurements of biotransformation rates in primary fish hepatocytes can be scaled up to the whole animal and thereby can provide data used in computational models to predict chemical bio-concentration factors in animals (K.A. Faye et al. 2014, I. Bischof et al. 2016). These and other supporting research results lead to the draft of an OECD Test Guideline on the determination of intrinsic clearance using cryopreserved rainbow trout hepatocytes and liver S9 subcellular fractions.

    Primacyt now offers cryopreserved pools of primary hepatocytes from rainbow trout kept under controlled conditions at Fraunhofer Institute for Marine Biotechnology and Cell Technology for at least two weeks in continuous flow water. Each lot is comprehensively tested for high viability and Cytochrome P450/Phase I and Phase II activities.

    Literature:
    K.A. Faye, R.T. Mingoia, I. Göritz, D.L. Nabb, A.D. Hoffman, B.D. Ferrel, H.M. Peterson, J.W. Nichols, H. Segner, and X. Han: Intra- and Interlaboratory Reliability of a Cryopreserved Trout Hepatocyte Assay for the Prediction of Chemical Bioaccumulation Potential. Environmental Science & Technology 2014, 48, 8170-8178

    I. Bischof, J. Köster, H. Segner, and C. Schlechtriem: Hepatocytes as in vitro test system to investigate to investigate metabolite patterns of pesticides in farmed rainbow trout and common carp: Comparison between in vivo and in vitro and across species. Comparative Biochemistry and Physiology, Part C, 2016, 187, 62-73




    Product Code CCHCP - Carp Hepatocytes (Common Carp) Crypreserved Plateable - Lot and Order Information

    - 2021-0203

    Lot /
    Gender

    Viability after isolation (%)

    Inventory (Number of vials)

    Viability after Thawing (%)

    Recovery (Viable number of cells/vial)

    Total number of cells available

    Viability (%) after shaking for 2 h at 14°C

    EUR per Vial

    CCH
    200504 / unknown

    98

    18

    98,2

    > 2 Mio

    > 36 Mio

    97,3

    1.200,00

    Q


    Quantity:

    Download Lot Information





    Product Code CCHCS - Carp Hepatocytes (Common Carp) Crypreserved for Use in Suspension - Lot and Order Information

    - 2021-0203

    Lot /
    Gender

    Viability after isolation (%)

    Inventory (Number of vials)

    Viability after Thawing (%)

    Recovery (Viable number of cells/vial)

    Total number of cells available

    Viability (%) after shaking for 2 h at 14°C

    EUR per Vial

    CCH
    200512 / mixed, pool of 3 (2f, 1 m)

    93

    12

    87,0

    >2 Mio

    > 24 Mio

    96,2

    1.200,00

    Q

    Quantity:


    Download Lot Information