Primacyt Transporter Assays - ADME - Toxicology Assays


Organic Anion Transporting

The organic anion transporting polypeptides (OATPs) of the SLCO gene family are expressed in many human organs. The uptake or the inhibition potential of compounds can be characterized with OATP1B1, OATP1B3, OATP1A2 and OATP2B1 expressed in HEK-293 cells.


Organic Cation

The organic cation transporters of the SLC family 22 are responsible for the uptake of cations or weak bases. The expression of human OCT1 (SLC22A1) and OCT2 (SLC22A2) is highly restricted to liver and kidney, respectively, while OCT3 (SLC22A3) is more widely distributed.


Na+-taurocholate co-transporting Polypeptides

The only liver-expressed Na+-taurocholate co-transporting polypeptide (NTCP) transports bile salts and sulfated compounds predominantly in a sodium-dependent manner. NTCP is expressed in the sinusoidal membrane of hepatocytes


Organic Anion

The organic anion transporters are part of SLC family 22. They are highly involved in renal and hepatic drug transport. OAT2 is expressed in the basolateral membrane of proximal tubules and in the sinusoidal membrane of hepatocytes, while OAT3 is primarily expressed in the kidney and plays a critical role in renal drug transport.


Apical sodium-dependent Bile Acid Transporters

Apical sodium-dependent bile acid transporter (ASBT, also called IBAT, ISBT: gene symbol SLC10A2)) is a Na+- dependent uptake transporter of bile acids and play a important role in the enterohepatic recirculation of bile acids. ASBT is expressed at apical membrane of ileal enterocytes, proximal renal tubule cells and gallbladder epithelial cells. With a reduced protein activity may result in the manifestation of a variety of gastrointestinal disorders.

Brief Summary

The knowledge of drug affinity and drug-drug interaction (DDI) to uptake and efflux transporters is a fundamental requirement in drug development, recommended by the U.S. Food and Drug Administration (FDA) and the European Medicines Agency (EMA).

Primacyt utilizes HEK-293 cells, each cell line is stable transfected with one uptake transporter protein. Our assays deliver information on the uptake, efflux and inhibitory potential of drugs and other compounds.