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Fresh Göttingen Minipig Hepatocytes from PRIMACYT


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Our fresh Göttingen Minipig hepatocytes are of highest quality. The level of quality insurance is identical with human hepatocytes. Typically we offer highest viability and functionality levels. In combination with appropriate cell morphology, our cell technology is one instrument for adequate in vitro - in vivo correlations for your purposes.

Characterization methods of fresh animal hepatocytes include the monitoring of liver specific functions, activities of drug transporters and the induction of cytochrome P450 activities. We also monitor and document cell morphology, attachment efficiency, monolayer confluency for platable cells and viability stability over time.



Göttingen Minipig hepatocytes are available in suspension and in multi-well plates (6-, 12-, 24- and 96-well plate). Our Primary Animal hepatocytes are available in suspension. Minimum order is 10 million viable cells. Suspension cells are for immediate use after arrival.
  • Products in SuspensionSuspensions will be sent on crushed ice on the day of isolation and should be used on the day of receipt. Included with each suspension order are following documents: Animal demographics (Göttingen Minipig) as datasheet, instructions for recovery of cells. Although hepatocytes in suspension are normally platable within 20-24 hours after isolation, we do not guarantee the platability of these cells, and recommend to order plated hepatocytes if this is the intended use.
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  • Plated ProductsPlated products will be sent at ambient temperature on day 1 after isolation and should be stored at 37°C with 95% humidity and 5% CO2 atmosphere. Included with each plated order are following documents: Animal Demographics (Göttingen Minipig), datasheet (with microscopic images of cells prior to shipping), manual for culture of primary hepatocytes.
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Fresh Göttingen Minipig Hepatocytes in Suspension

Biohazard warning: Tissue fractions such as hepatocytes should be considered as potentially biohazardous, and should be treated as biohazards in the laboratory.
Only for research purposes. Not for use in human diagnostics or therapeutics.

Product No.
Product
Specification
Packaging
EUR per 1 Mio Cells
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GMHS-01

In suspension

> 10 Mio viable cells

Vial

59,00

> 100 Mio viable cells

Vial

53,10

> 200 Mio viable cells

Vial

47,20








Fresh Minipig Hepatocytes Plated


It is recommended to culture Göttingen Minipig hepatocytes on collagen coated culture plates in HHMM (Human Hepatocyte Maintenance Medium), a serum-free culture medium containing Hepatocyte Growth Factor and Epidermal Growth Factor.
Cells in plates require light protected storage at 37 ℃ and 5 % CO2 .

Product No.
Product
Specification
Packaging
EUR per Plate
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GMHP-06

6 well plate

1.2 Mio cells per well

1-2 plates

515,00

Collagen coated

1.2 Mio cells per well

3-5 plates

465,00

1.2 Mio cells per well

6-10 plates

390,00

1.2 Mio cells per well

> 10 plates

360,00


Product No.
Product
Specification
Packaging
EUR per Plate
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GMHP-12

12 well plate

600.000 cells per well

1-2 plates

515,00

Collagen coated

600.000 cells per well

3-5 plates

465,00

600.000 cells per well

6-10 plates

390,00

600.000 cells per well

> 10 plates

360,00


Product No.
Product
Specification
Packaging
EUR per Plate
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GMHP-24

24 well plate

300.000 cells per well

1-2 plates

515,00

Collagen coated

300.000 cells per well

3-5 plates

465,00

300.000 cells per well

6-10 plates

390,00

300.000 cells per well

> 10 plates

360,00


Product No.
Product
Specification
Packaging
EUR per Plate
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Put to Basket

GMHP-96

96 well plate

50.000 cells per well

1-2 plates

515,00

Collagen coated

50.000 cells per well

3-5 plates

465,00

50.000 cells per well

6-10 plates

390,00

50.000 cells per well

> 10 plates

360,00


Biohazard warning: Tissue fractions such as hepatocytes should be considered as potentially biohazardous, and should be treated as biohazards in the laboratory.
Only for research purposes. Not for use in human diagnostics or therapeutics.




More Information

Fig 1: Göttingen Minipig hepatocytes at day 3 of culture in 24well plates cultured with HHMM (left: fresh Minipig hepatocytes, right: cryopreserved Minipig hepatocytes)..

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Hepatocytes are the most prominent cells of the liver. They synthesize albumin, produce urea and eliminate toxic substances from the blood. In this biotransformation process transporter proteins (influx and efflux transporter) and phase I+II reactions play a central role. Primary hepatocytes are perfectly suited for in vitro metabolism and toxicity studies prior to preclinical tests. Minipig hepatocytes are isolated from livers obtained from male or female Göttingen Minipigs and are available fresh in suspensions or in various culture formats (6, 12, 24 and 96well) as well as cryopreserved cells. We recommend to culture Minipig hepatocytes on collagen coated culture plates in PRIMACYT’s serum-free HHMM (Human Hepatocyte Maintenance Medium). Hepatocyte specific morphology and cytochrome P450 protein activities are maintained and/or remain inducible under these culture conditions (Fig. 1 and 2).


Fig 2: Induction of Ethoxyresorufin-O-deethylase (EROD) activity by ß-Naphthoflavone (ß-NF) for 48 h in Göttingen Minipig hepatocytes cultured with HHMM (left: fresh hepatocytes, induction day 3-5; right: cryopreserved hepatocytes, induction day 1-3 and day 5-7).
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