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Benefit from PRIMACYT's Expertise as European Reference Laboratory for Alternatives to Animal Testing

Validate your Preclinical Drug Development with PRIMACYT HEK293 Transporter Assay Studies

The knowledge of drug affinity and drug-drug interaction (DDI) to organic anion transporting polypeptides (OATPs) and other transporter proteins like P-glycoprotein (P-gp, MDR1, ABCB1) is a basic requirement in drug development and is also recommended by the U.S. Food and Drug Administration (FDA) and the European Medicines Agency (EMA).

OATPs of the SLCO (former SLC21) superfamily are of fundamental importance in the transport of drugs across cell membranes, e.g. in intestine, liver, kidney, brain, skeleton muscle and placenta.
HEK293 cells are easy to grow in culture and transfection kits are commercially available. Thus, HEK293 is an excellent cell line to use in transfection experiments, or to produce recombinant DNA or gene products. This has made them a popular research tool in cell biology studies.

The typical workflow is the transfection of a protein and the subsequent analysis of the protein expression.

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PRIMACYT HEK 293 Transporter Assays for Basic R&D and Drug Development.


The HEK293 and MDCK2 cell lines utilised by Primacyt have been generated by the Department of Clinical Pharmacology at the University Medicine of Greifswald. The Department of Clinical Pharmacology also provided an in depth characterisation of the cell lines using Western blot, immunohistochemical assays, an LC-MS/MS based analytics of protein expression and radioactive assays to determine uptake and efflux functions of the transporters.

In addition the transporter function was characterized by fluorescence substrates at Primacyt. We do now have a panel of well characterized stable transfected HEK293 cells expressing different transporter proteins that are used to study uptake of drugs and chemicals in vitro.

HEK293 cells expressing transporter proteins for use in Drug Development

• Drug - Drug Interaction.
• Pharmacokinetics
• Absorption, distribution and excretion of drugs and other compounds.



Hepatic OATPs (OATP1B1, OATP1B3, OATP2B1) but also the sodium taurocholate co-transporting polypeptide (NTCP) are expressed at the sinusoidal membrane of human hepatocytes and transport several compounds into hepatocytes for biotransformation. In intestine, absorption of several compounds is mediated by e.g. OATP2B1 and the bile acid transporter ASBT (apical sodium-dependent bile salt transporter) which both are expressed at the brush border membrane.


The knowledge of drug affinity and drug-drug interaction (DDI) and the role of organic anion
transporting polypeptides (OATPs) and other transporter proteins like P-glycoprotein (MDR1,
P-gp, ABCB1) is a basic requirement in drug development and is also recommended by the
U.S. Food and Drug Administration (FDA) and the European Medicines Agency (EMA).
OATPs of the SLCO (former SLC21) superfamily are of fundamental importance in the
transport of drugs across cell membranes, e.g. in intestine, liver, kidney, brain, skeleton
muscle and placenta.

Hepatic OATPs (OATP1B1, OATP1B3, OATP2B1) but also the sodium taurocholate cotransporting
polypeptide (NTCP) are expressed at the sinusoidal membrane of human
hepatocytes and transport several compounds into hepatocytes for biotransformation. In
intestine, absorption of several compounds is mediated by e.g. OATP2B1 and the bile acid
transporter ASBT (apical sodium-dependent bile salt transporter) which both are expressed
at the brush border membrane.

Primacyt utilizes well characterized stable transfected human embryonic kidney cells
(HEK293) expressing different transporter proteins to study uptake of drugs and chemicals in
vitro.




Contact us also for Studies of Metabolism - Toxicity - Cytochrome P450 induction - Hepatocellular Function







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